DCPS Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81749

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DCPS Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASGDGDGEDAVVILEKTPFQVEQVAQLLTGSPE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (82%). Human reactivity reported in scientific literature (PMID: 25712129).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DCPS Antibody - BSA Free

Western Blot: DCPS Antibody [NBP1-81749]

Western Blot: DCPS Antibody [NBP1-81749]

Western Blot: DCPS Antibody [NBP1-81749] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-DCPS antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: DCPS Antibody [NBP1-81749]

Immunocytochemistry/ Immunofluorescence: DCPS Antibody [NBP1-81749]

Immunocytochemistry/Immunofluorescence: DCPS Antibody [NBP1-81749] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Western Blot: DCPS Antibody [NBP1-81749]

Western Blot: DCPS Antibody [NBP1-81749]

Western Blot: DCPS Antibody [NBP1-81749] - Analysis in human cell line PC-3.
DCPS Antibody - BSA Free Immunohistochemistry-Paraffin: DCPS Antibody [NBP1-81749]

Immunohistochemistry-Paraffin: DCPS Antibody [NBP1-81749]

Staining of human liver shows strong nuclear positivity in hepatocytes.

Applications for DCPS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
WB, ICC/IF reported in scientific literature (PMID: 25712129). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DCPS

Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides

Alternate Names

DCS1, DCS-1, decapping enzyme, scavenger, EC 3.-, heat shock-like protein 1, HINT5, HINT-5homolog of C. elegans 7meGMP-directed hydrolase dcs-1, Hint-related 7meGMP-directed hydrolase, Histidine triad protein member 5, HSL1, HSPC015, mRNA decapping enzyme, scavenger mRNA-decapping enzyme DcpS

Gene Symbol

DCPS

Additional DCPS Products

Product Documents for DCPS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DCPS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DCPS Antibody - BSA Free

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Protocols

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FAQs

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