Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 335 to 385 of human DNA-Damage Inducible 1 Homolog 2 using the numbering given in entry NP_115717.3 (GeneID 84301).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DDI2 Antibody - BSA Free
Western Blot: DDI2 Antibody [NBP2-36795]
Western Blot: DDI2 Antibody [NBP2-36795] - Detection of human DDI2 by western blot of immunoprecipitates. Sample: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDI2 antibody NBP2-36795 used for IP at 6 ug per reaction. Detection: Chemiluminescence with an exposure time of 30 seconds.Western Blot: DDI2 Antibody [NBP2-36795]
Western Blot: DDI2 Antibody [NBP2-36795] - Detection of human DDI2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDI2 antibody NBP2-36795 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for DDI2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DDI2
Alternate Names
DDI1, DNA-damage inducible 1, homolog 2, DDI1, DNA-damage inducible 1, homolog 2 (S. cerevisiae), DNA-damage inducible 1 homolog 2 (S. cerevisiae), DNA-damage inducible protein 2 (DDI2), MGC14844, protein DDI1 homolog 2, RP4-680D5.5
Gene Symbol
DDI2
UniProt
Additional DDI2 Products
Product Documents for DDI2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DDI2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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