DDT Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13904

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LALGQDRILIRFFPLESWQIGKIGTVMT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDT Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DDT Antibody [NBP2-13904]

Immunocytochemistry/ Immunofluorescence: DDT Antibody [NBP2-13904]

Immunocytochemistry/Immunofluorescence: DDT Antibody [NBP2-13904] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: DDT Antibody [NBP2-13904]

Immunohistochemistry-Paraffin: DDT Antibody [NBP2-13904]

Immunohistochemistry-Paraffin: DDT Antibody [NBP2-13904] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Applications for DDT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DDT

D-dopachrome decarboxylase, also known as D-dopachrome tautomerase (DDT), phenylpyruvate tautomerase II and DDCT, is a member of the migration inhibitory factor (MIF) family. DDT, via tautomerization and decarboxylation, converts D-dopachrome into 5,6-dihydroxyindole (DHI). DDT may also play a role in melanin biosynthesis.

Long Name

D-Dopachrome Tautomerase

Alternate Names

D-Dopachrome Decarboxylase, DDCT, DOPD, MIF2, Phenylpyruvate Tautomerase II

Gene Symbol

DDT

Additional DDT Products

Product Documents for DDT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DDT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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