DEC1 Antibody (5B1) - Azide and BSA Free

Novus Biologicals | Catalog # H00008553-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5B1

Format

Azide and BSA Free
View all DEC1 Primary Antibodies »

Product Specifications

Immunogen

BHLHB2 (NP_003661, 130 a.a. ~ 229 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LSGRNVETGQEMFCSGFQTCAREVLQYLAKHENTRDLKSSQLVTHLHRVVSELLQGGTSRKPSDPAPKVMDFKEKPSSPAKGSEGPGKNCVPVIQRTFAH

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

BHLHB2 - basic helix-loop-helix domain containing, class B, 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DEC1 Antibody (5B1) - Azide and BSA Free

Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHB2 expression in NIH/3T3.
Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - Western Blot analysis of BHLHB2 expression in Hela NE ( Cat # L013V3 ).
Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1 Analysis of BHLHB2 expression in HepG2.
Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHB2 expression in Raw 264.7.
Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01]

Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHE40 expression in K-562.
ELISA: DEC1 Antibody (5B1) [H00008553-M01]

ELISA: DEC1 Antibody (5B1) [H00008553-M01]

ELISA: DEC1 Antibody (5B1) [H00008553-M01] - Detection limit for recombinant GST tagged BHLHB2 is approximately 0.03ng/ml as a capture antibody.

Applications for DEC1 Antibody (5B1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DEC1

DEC1 encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. Differentiated embryo chondrocyte expressed gene 1 is believed to be involved in the control of cell differentiation.

Alternate Names

basic helix-loop-helix domain containing, class B, 2, basic helix-loop-helix family, member e40, bHLHb2, bHLHe40, Class B basic helix-loop-helix protein 2, class E basic helix-loop-helix protein 40, DEC1HLHB2, differentially expressed in chondrocytes 1, Differentially expressed in chondrocytes protein 1, differentiated embryo chondrocyte expressed gene 1, Enhancer-of-split and hairy-related protein 2, SHARP2, SHARP-2, Stimulated by retinoic acid gene 13 protein, STRA13FLJ99214, Stra14

Entrez Gene IDs

8553 (Human)

Gene Symbol

BHLHE40

OMIM

604256 (Human)

UniProt

NP_003661 (Human)

Additional DEC1 Products

Product Documents for DEC1 Antibody (5B1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DEC1 Antibody (5B1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DEC1 Antibody (5B1) - Azide and BSA Free

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