DermaTACs In Situ Apoptosis Detection Kit

Catalog # Availability Size / Price Qty
4829-30-K
Product Details
Citations (5)
FAQs
Reviews (1)

DermaTACs In Situ Apoptosis Detection Kit Summary

Provides researchers with an effective method for measuring apoptosis in skin samples using DNA end-labeling using terminal deoxynucleotidyl transferase (TdT), modified nucleotides (BrdU).

Key Benefits

• Performance tested on dermal tissues.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Helps resolve unique problems encountered when detecting apoptosis in dermal tissue.

Why Use the DermaTACS In Situ Apoptosis Detection Kit?

The DermaTACS Kit was developed to provide researchers with an effective method for measuring apoptosis in skin samples. The kit was based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT), modified nucleotides (BrdU). Detection of incorporated molecules is achieved using a biotinylated BrdU antibody and chromogenic substrate with a Streptavidin horseradish peroxidase detection system.

The extracellular scaffolding in skin tissue can make it problematic to permeabilize and high background is a common problem with skin samples. The DermaTACS Kit includes two permeabilization reagents as options for permeabilization along with a detailed protocol with hints and tips for optimal labeling of skin samples based on empirical testing. Low background is achieved using a combination of optimized reagents and visualization methods. Also included is TACS-Nuclease to generate a positive control from your own sample. In conjunction with the excellent contrast between the TACS Blue Label and the Red Counterstain C, the positive control ensures easy data interpretation.

Kit Contents

• Streptavidin-HRP
• TACS-Blue Label
• TACS-Nuclease
• TACS-Nuclease Buffer
• Red Counterstain C
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TdT Enzyme
• B-dNTP Mix
• anti-BrdU antibody
• Strep-Diluent
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Citations for DermaTACs In Situ Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. ARID1A Mutations Promote P300-Dependent Endometrial Invasion through Super-Enhancer Hyperacetylation
    Authors: MR Wilson, JJ Reske, J Holladay, S Neupane, J Ngo, N Cuthrell, M Wegener, M Rhodes, M Adams, R Sheridan, G Hostetter, FT Alotaibi, PJ Yong, MS Anglesio, BA Lessey, RE Leach, JM Teixeira, SA Missmer, AT Fazleabas, RL Chandler
    Cell Rep, 2020;33(6):108366.  2020
  2. Ethanol consumption synergistically increases ultraviolet radiation induced skin damage and immune dysfunction
    Authors: RM Brand, JM Stottlemye, MC Paglia, CD Carey, LD Falo
    J Dermatol Sci, 2020;0(0):.  2020
  3. Tumor-Preferential Induction of Immune Responses and Epidermal Cell Death in Actinic Keratoses by Ingenol Mebutate
    PLoS ONE, 2016;11(9):e0160096.  2016
  4. Chemotherapy targets the hair-follicle vascular network but not the stem cells.
    Authors: Amoh Y, Li L, Katsuoka K, Hoffman RM
    J. Invest. Dermatol., 2007;127(1):11-5.  2007
  5. Targeted deletion of RasGRP1 impairs skin tumorigenesis.
    Authors: Sharma A, Fonseca L, Rajani C, Yanagida J, Endo Y, Cline J, Stone J, Ji J, Ramos J, Lorenzo P
    Carcinogenesis, 0;35(5):1084-91.  0

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DermaTACs In Situ Apoptosis Detection Kit
By Anonymous on 04/13/2018
Application:

This kit was ordered from Trevigen (order number 64163) and used a few years ago. It was the positive control using Proteinase K and TACS-Nuclease in mouse skin.