Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 648538
View all DGK-iota Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DGK‑ iota
Ser925-Val1065 (predicted)
Accession # O75912

Specificity

Detects human and mouse DGK‑ iota in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human DGK-alpha, -beta, -delta, -epsilon, -eta, -gamma, -kappa, -theta, or -zeta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for DGK-iota Antibody (648538) [FITC]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: DGK-iota

Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-alpha (80 kDa), -beta (90 kDa), and - gamma (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-delta (130 kDa) and DGK-

Long Name

Diacylglycerol Kinase, iota 64kDa

Alternate Names

DGKI, DGKiota

Gene Symbol

DGKI

Additional DGK-iota Products

Product Documents for DGK-iota Antibody (648538) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DGK-iota Antibody (648538) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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FAQs

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