Detects human and mouse DGK‑ iota in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human DGK-alpha, -beta, -δ, -epsilon, -eta, -gamma, -kappa, -theta, or -zeta is observed.
Monoclonal Mouse IgG2A Clone # 648538
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human DGK‑ iota Ser925-Val1065 (predicted) Accession # O75912
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse DGK‑ iota by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and mouse brain tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat DGK‑ iota Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6435) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK‑ iota at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Diacylglycerol kinase iota (DGK-iota ) is an approximately 110 kDa kinase that phosphorylates the lipid
diacylglycerol. DGK-iota also inhibits RasGRP3 and limits Rap1 activation. It contains
two zinc finger regions (aa 178-232 and aa 251-310), a catalytic domain (aa
372-507), and two ankyrin repeats (aa 958-990 and aa 997-1026). Over aa
925-1065, human DGK-iota shares 94% and 96% aa sequence identity with mouse
and rat DGK-iota, respectively.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.