Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DHPS Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 319 to 369 of human Deoxyhypusine Synthase using the numbering given in entry NP_001921.1 (GeneID 1725).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHPS Antibody - BSA Free

Western Blot: DHPS Antibody [NBP2-32256]

Western Blot: DHPS Antibody [NBP2-32256]

Western Blot: DHPS Antibody [NBP2-32256] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-DHPS antibody NBP2-32256 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: DHPS Antibody [NBP2-32256]

Immunoprecipitation: DHPS Antibody [NBP2-32256]

Immunoprecipitation: DHPS Antibody [NBP2-32256] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-DHPS antibody NBP2-32256 used for IP at 6 ug per reaction. For blotting immunoprecipitated DHPS, NBP2-32256 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DHPS Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:500 - 1:2500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DHPS

The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A. In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. This gene consists of nine exons spanning 6.6 kb. Three transcript variants have been isolated. However, only transcript variant 1 encodes an active protein. The shorter variants may act as modulating factors of DHPS activity.

Alternate Names

deoxyhypusine synthase, DHS, DS, EC 2.5.1, EC 2.5.1.46, hypusine, MIG13, migration-inducing gene 13

Gene Symbol

DHPS

UniProt

NP_001921.1 (Human)

Additional DHPS Products

Product Documents for DHPS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DHPS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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