Digoxigenin Antibody - BSA Free

Novus Biologicals | Catalog # NB100-65495

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Non-species specific

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunofluorescence, In-situ Hybridization

Cited:

In Situ Hybridization

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

BSA Free
View all Digoxigenin Primary Antibodies »

Product Specifications

Immunogen

Digoxin-3-CM conjugated to keyhole limpet haemocyanin

Specificity

Recognizes Digoxigenin, a steroid found in plants that is often attached to oligonucleotides and used as a molecular probe to detect mRNA production. This cross reacts with Digoxin and Digitoxin. Negligible reactivity has been observed with Ouabain.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Digoxigenin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:5000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Use in In-situ Hybridization reported in scientific literature (PMID:31767540)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

5.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Digoxigenin

Digoxigenin is a small organic molecule rather than amino acids. DIG can be covalently added to proteins or nucleic acids which makes it very handy in its diverse applications.

Alternate Names

DIG

Additional Digoxigenin Products

Product Documents for Digoxigenin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Digoxigenin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Digoxigenin Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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