Dihydrokainic acid

Tocris Bioscience | Catalog # 0111

EAAT2 (GLT-1)-selective non-transportable inhibitor of L-glutamate and L-aspartate uptake
Tocris Bioscience

Key Product Details

Description

EAAT2 (GLT-1)-selective non-transportable inhibitor of L-glutamate and L-aspartate uptake
View all Glutamate (EAAT) Transporter Inhibitors »

Product Description

Dihydrokainic acid is a selective EAAT2(GLT1) non-transportable inhibitor of L-glutamate and L-aspartate uptake (Ki = 23 μM). Dihydrokainic acid is 130-fold selective over EAAT1 and EAAT3 (Ki > 3 mM). In [3H]-d-Asp uptake assays in HEK293 cells expressing human EAAT2, EAAT1 and EAAT3, Ki values are 89 μM, > 3 mM and > 3 mM, respectively. Respective Km values in a FLIPR Membrane Potential (FMP) assay, are 31 μM, > 3 mM and > 3 mM

Product Specifications for Dihydrokainic acid

Molecular Weight

215.25

Formula

C10H17NO4

Storage

Store at RT

Chemical Name

(2S,3S,4R)-2-Carboxy-4-isopropyl-3-pyrrolidineacetic acid

CAS Number

52497-36-6

PubChem ID

107883

InChI Key

JQPDCKOQOOQUSC-OOZYFLPDSA-N

SMILES

O=C(C[C@H]1[C@@H]([C@H](C)C)CN[C@@H]1[C@](O)=O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 5.38 25

Preparing Stock Solutions for Dihydrokainic acid

The following data is based on the product molecular weight 215.25.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.25 mM 18.58 mL 92.92 mL 185.83 mL
1.25 mM 3.72 mL 18.58 mL 37.17 mL
2.5 mM 1.86 mL 9.29 mL 18.58 mL
12.5 mM 0.37 mL 1.86 mL 3.72 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 83 publications citing the usage of this product.

Product Documents for Dihydrokainic acid

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Dihydrokainic acid

For research use only

Citations for Dihydrokainic acid

Customer Reviews for Dihydrokainic acid (1)

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  • Works as expected.
    Name: Dipa Pati
    Species: Mouse
    Assay Type: Ex Vivo
    Cell Line/Tissue: Bed nucleus of the stria terminalis
    Verified Customer | Posted 05/26/2020
    We performed ex-vivo slice physiology on Bed nucleus of the stria terminalis (BNST) slices. We bath applied DHK (300 micromolar) on a BNST neuron in the current-clamp mode. DHK depolarized the neuron and the drug effect washed out.
    Dihydrokainic acid 0111

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