Key Product Details

Species Reactivity

Porcine

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Dihydrolipoamide Dehydrogenase/DLD Primary Antibodies »

Product Specifications

Immunogen

Dihydrolipoamide Dehydrogenase/DLD [Porcine Heart] (Uniprot: P09623)

Reactivity Notes

Cross reactivity against Dihydrolipoamide Dehydrogenase/DLD from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-77778]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-77778]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-77778] - Western Blot of Rabbit Dihydrolipoamide Dehydrogenase/DLD antibody. Lane 1: Dihydrolipoamide Dehydrogenase/DLD Reduced [0.05ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Dihydrolipoamide Dehydrogenase/DLD Non-Reduced [0.05ug]. Primary Antibody: Anti-Dihydrolipoamide Dehydrogenase/DLD at 1:1000 overnight at 2-8C. Secondary Antibody: Goat anti-Rabbit IgG [H&L] HRP at 1:40,000 for 30mins at RT. Blocking: Blocking buffer for 1hr at RT. Expected MW: 55kDa. Exp: 10 secs.

Applications for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by ELISA and western blot and is assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:30,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dihydrolipoamide Dehydrogenase/DLD

Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Alternate Names

Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3

Gene Symbol

DLD

UniProt

P09623 (Human)

Additional Dihydrolipoamide Dehydrogenase/DLD Products

Product Documents for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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