Dkk-4 Antibody

Novus Biologicals | Catalog # NB100-1000

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Dkk-4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence RQHARLRVCQKIEKL corresponding to C-Terminus according to NP_055235.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Dkk-4 Antibody

Western Blot: Dkk-4 Antibody [NB100-1000] -

Western Blot: Dkk-4 Antibody [NB100-1000] - (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for Dkk-4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 30-35 kDa band observed in Jurkat lysate. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa band [form (i)], 30-32 kDa band [form (ii)] and 15-17 kDa band [form (iii)]. We believe will recognise 30-32 kDa band species of DKK4.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dkk-4

DKK4 is a secreted protein that is a member of the dickkopf family. Like some other members of the dickkopf family DKK4 contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The activity of DKK4 is modulated by binding to the Wnt co-receptor and the co-factor kremen 2, and it is proposed that it is also an inhibitor of the Wnt signaling pathway.

Long Name

Dickkopf-4

Alternate Names

Dkk4

Entrez Gene IDs

27121 (Human)

Gene Symbol

DKK4

UniProt

Q9UBT3, NP_055235.1 (Human)

Additional Dkk-4 Products

Product Documents for Dkk-4 Antibody

Certificate of Analysis

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Download SDS
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Product Specific Notices for Dkk-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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