DLG7/HURP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87976

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DLG7/HURP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASRHRKDISTEMIRTKIAHRKSLSQKENRHKEYERNRHFGLKDVNIPTLEGRILVELDETSQGLVPEKTNVKPRAMKTILGDQRKQMLQKYKEEKQLQKLKEQREKAKRGIFKVGRYRPDMPCFLLSNQNAVKAE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DLG7/HURP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DLG7/HURP Antibody [NBP1-87976]

Immunocytochemistry/ Immunofluorescence: DLG7/HURP Antibody [NBP1-87976]

Immunocytochemistry/Immunofluorescence: DLG7/HURP Antibody [NBP1-87976] - Staining of human cell line A-431 shows localization to cytosol & microtubule organizing center. Antibody staining is shown in green.
DLG7/HURP Antibody - BSA Free Western Blot: DLG7/HURP Antibody - BSA Free [NBP1-87976]

Western Blot: DLG7/HURP Antibody - BSA Free [NBP1-87976]

Analysis in human cell lines U2OS and SK-MEL-30 using Anti-DLGAP5 antibody. Corresponding DLGAP5 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Analysis in human testis and liver tissues using NBP1-87976 antibody. Corresponding DLGAP5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NBP1-87976] - Staining of human pancreas shows no positivity in exocrine or endocrine glandular cells as expected.

Applications for DLG7/HURP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DLG7/HURP

DLG7/HURP is a novel component of the Ran-importin spindle assembly pathway. DLG7/HURP binds microtubules and affects their organization both in vitro and in vivo. In egg extract, anti-DLG7/HURP antibodies disrupt the formation of both Ran-dependent and chromatin and centrosome-induced spindles. DLG7/HURP is also required for the proper formation and function of mitotic spindles in HeLa cells. DLG7/HURP is also over-expressed in some tumor types

Alternate Names

DAP-5, Discs large homolog 7, discs, large (Drosophila) homolog-associated protein 5, disks large-associated protein 5, DLG7, Hepatoma up-regulated protein, HURPDLG1, KIAA0008discs, large homolog 7 (Drosophila), large homolog 7

Gene Symbol

DLGAP5

Additional DLG7/HURP Products

Product Documents for DLG7/HURP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DLG7/HURP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DLG7/HURP Antibody - BSA Free

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Protocols

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