DNA Adenine Methylase Antibody (RB1) - BSA Free

Novus Biologicals | Catalog # NBP2-66588

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # RB1

Format

BSA Free
View all DNA Adenine Methylase Primary Antibodies »

Product Specifications

Immunogen

Hybridoma obtained after immunization of mice with recombinant DNA Adenine Methylase protein.

Reactivity Notes

Gram Negative Bacteria

Specificity

The antibody reacts with the recombinant protein as produced by E.coli. We have no data to support to its reactivity with wild type DAM protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for DNA Adenine Methylase Antibody (RB1) - BSA Free

Application
Recommended Usage

Western Blot

1-5 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNA Adenine Methylase

DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.

Alternate Names

DAM, DAM methylase

UniProt

P0AEE8 (E. coli)

Additional DNA Adenine Methylase Products

Product Documents for DNA Adenine Methylase Antibody (RB1) - BSA Free

Certificate of Analysis

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Product Specific Notices for DNA Adenine Methylase Antibody (RB1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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