DNMT3B Antibody

Name: DNMT3B Antibody
Brand: Novus Biologicals
SKU: NBP3-18646
Price: 129 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-18646

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

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Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein (1).

Clonality

Polyclonal

Host

Rabbit

Description

Novus Biologicals Rabbit DNMT3B Antibody (NBP3-18646) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DNMT3B Antibody

Western Blot: DNMT3B Antibody [NBP3-18646]

Western Blot: DNMT3B Antibody [NBP3-18646] - Figure 2. Western blot analysis using the antibody directed against DNMT3B Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the MW marker (in kDa) is shown on the left.

ELISA: DNMT3B Antibody [NBP3-18646]

ELISA: DNMT3B Antibody [NBP3-18646] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse DNMT3B in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,500.

Applications for DNMT3B Antibody

Application

Recommended Usage

ELISA

1:100-1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: DNMT3B

DNA (cytosine-5)-methyltransferase 3B (DNAMT3b) is a C5-methyltransferase family member that is required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development.

Improper methylation patterns have been linked to certain human tumors and developmental abnormalities. DNMT3b antibodies are useful tools for DNA methlyation research and studies on certian cancer.

Long Name

DNA [Cytosine-5-]-Methyltransferase 3B

Alternate Names

ICF1, M.HsaIIIB

Gene Symbol

DNMT3B

UniProt

Q9UBC3 (Human)

Additional DNMT3B Products

Product Documents for DNMT3B Antibody

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Certificate of Analysis

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Product Specific Notices for DNMT3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis

Epigenetics

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