DOCK11 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-81662

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DOCK11 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human dedicator of cytokinesis 11 using the numbering given in entry NP_653259.3 (GeneID 139818).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DOCK11 Antibody - BSA Free

Immunoprecipitation: DOCK11 Antibody [NB100-81662]

Immunoprecipitation: DOCK11 Antibody [NB100-81662]

Immunoprecipitation: DOCK11 Antibody [NB100-81662] - Detection of human DOCK11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK11 antibody NB100-81662 used for IP at 3 ug per reaction. DOCK11 was also immunoprecipitated by rabbit anti-DOCK11 antibody NB100-81663. For blotting immunoprecipitated DOCK11, NB100-81662 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for DOCK11 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Not recommended for western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DOCK11

DOCK11 (dedicator of cytokinesis 11) is also known as zizimin-2. It is a DOCK180-related guanine nucleotide exchange factor (GEF) for the small GTPase, Cdc42. As a GEF, DOCK11 functions to catalyze the dissociation of GDP from the GTPase. Dissociation of GDP allows an exchange for GTP and subsequent downstream signaling by the GTPase. Alternate names for DOCK11 include activated Cdc42-associated guanine nucleotide exchange factor, ACG, and ZIZ2.

Alternate Names

ACG, Activated Cdc42-associated guanine nucleotide exchange factor, Activated Cdc42-associated guanine nucleotide exchange factor, ACG, bB128O4.1, bB128O4.1.1 (novel protein), dedicator of cytokinesis 11, FLJ32122, FLJ43653, ZIZ2dedicator of cytokinesis protein 11, zizimin2, zizimin-2

Entrez Gene IDs

139818 (Human)

Gene Symbol

DOCK11

UniProt

NP_653259.3 (Human)

Additional DOCK11 Products

Product Documents for DOCK11 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DOCK11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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