DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-71987
![Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]](https://resources.rndsystems.com/images/products/DOG1-TMEM16A-Antibody-OTI1C8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71987-img0006.jpg "Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1C8
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-333 of human ANO1 (NP_060513) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]](https://resources.rndsystems.com/images/products/DOG1-TMEM16A-Antibody-OTI1C8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71987-img0001.jpg "Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]")
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]](https://resources.rndsystems.com/images/products/DOG1-TMEM16A-Antibody-OTI1C8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71987-img0002.jpg "Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]")
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]](https://resources.rndsystems.com/images/products/DOG1-TMEM16A-Antibody-OTI1C8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71987-img0003.jpg "Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]")
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]](https://resources.rndsystems.com/images/products/DOG1-TMEM16A-Antibody-OTI1C8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71987-img0004.jpg "Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]")
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987] - Analysis of Human embryonic cerebellum tissue.. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]](https://resources.rndsystems.com/images/products/DOG1-TMEM16A-Antibody-OTI1C8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71987-img0005.jpg "Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]")
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]](https://resources.rndsystems.com/images/products/DOG1-TMEM16A-Antibody-OTI1C8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71987-img0007.jpg "Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]")
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987]
Immunohistochemistry: DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free [NBP2-71987] - Analysis of Human testicular cancer tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: ANO1/TMEM16A
Long Name
Anoctamin 1
Alternate Names
DOG1, ORAOV2, TAOS2, TMEM16A
Gene Symbol
ANO1
Additional ANO1/TMEM16A Products
Product Documents for DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DOG1/TMEM16A Antibody (OTI1C8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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