Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
  (3 citations)
(3 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Sheep
  • Source
    Polyclonal Donkey IgG
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    NL557
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    Please refer to the PDF datasheet for specifications.
  • Immunohistochemistry
    Please refer to the PDF datasheet for specifications.
  • Immunocytochemistry
    Please refer to the PDF datasheet for specifications.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Role of Nesfatin-1 in the Reproductive Axis of Male Rat
    Sci Rep, 2016;6(0):32877.
    Application: IHC
  2. Bone marrow-derived multipotent stromal cells attenuate inflammation in obliterative airway disease in mouse tracheal allografts.
    Authors: Casey A, Dirks F, Liang O, Harrach H, Schuette-Nuetgen K, Leeman K, Kim C, Gerard C, Subramaniam M
    Stem Cells Int, 2014;2014(0):468927.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  3. Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjogren's-like disease.
    Authors: Khalili S, Liu Y, Kornete M, Roescher N, Kodama S, Peterson A, Piccirillo CA, Tran SD
    PLoS ONE, 2012;7(6):e38615.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Immunohistochemistry Reagents
Description Application Cat# Citations Images  

NorthernLights Guard Mounting Media

NL996
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB , Simple Western HAF016 16  
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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Average Rating: 4.3 (Based on 3 reviews)

Have you used Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody?

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Read what people are saying who have used Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody.

We have 2 reviews tested in 2 species: Human, Sheep.
We have 2 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Flow Cytometry.

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(1)
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(2)
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Human
(1)
Sheep
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All Species

Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody NL010 Array NL010
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Excellent
 ICC/IF Human Anonymous 09/27/2017
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Image Details
Immunocytochemistry/Immunofluorescence Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody NL010
Immunocytochemistry/Immunofluorescence: Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody [NL010].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested HUVEC human umbilical vein endothelial cells
Species Human
 Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody NL010 Array NL010
Enlarge
Very Good
  Anonymous 12/23/2016
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Image Details
 Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody NL010
: Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody [NL010].

Other Experimental Details

Other Experimental Details Used with the Human CD109 antibody from R&D Systems.
  Very Good
 Flow Sheep Anonymous 07/27/2016
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Summary

Application Flow Cytometry
Sample Tested Sheep IgG
Species Sheep

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