Detection of Dopa Decarboxylase/DDC by Western Blot. Western blot of bovine adrenal medulla lysate showing specific immunolabeling of the ~55 kDa DDC protein.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: Dopa Decarboxylase/DDC
DOPA decarboxylase (Aromatic-L-amino acid decarboxylase; also DDC) is a 54 kDa member of the alpha -family of pyroxidal phosphate enzymes. Functional DDC is a homodimeric, pyridoxal phosphate-dependent enzyme that calatyzes the decarboxylation of DOPA to dopamine, L-tryptophan to tryptamine, and 5-hydroxytryptophan to serotonin. Both dopamine and serotonin are important neurotransmitters. It also demonstrates deaminase activity, and is regulated by O2 availability. Bovine DDC is 487 amino acids (aa) in length and contains an N-terminal decarboxylase domain (aa 35-414) and a C-terminal pyroxidal binding site (L303). Bovine DDC is 88% and 86% aa identical to human and rat DDC, respectively.
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