DSPP Antibody - BSA Free

Western Blot: DSPP Antibody - Azide and BSA Free [NBP2-92546] -

Alkbh5 improved odontoblast differentiation of mDPC6T cells. (A) Transfer efficiency of overexpressed Alkbh5 in mDPC6T cells was detected and observed via red fluorescent protein labeling under a microscope (see above) after transfection for 72 h. The lower panel shows the immunofluorescence images taken simultaneously. Scale bar: 100 μm (original magnification × 100). (B,C)Alkbh5 expression level was measured via qPCR and western blotting in the Alkbh5 overexpression group and negative control group. (D) The protein levels of DSPP and DMP1 in the Alkbh5 overexpression group and control group were measured via western blotting after 7 and 14 days of odontoblast induction. (E) The mRNA expression levels of Dspp and Dmp1 were measured via qRT-PCR after 7 and 14 days of odontoblast induction. (F) ALP activity was determined. Alkbh5, AlkB homolog H5; Dspp, dentin sialophosphoprotein; Dmp1, dentin matrix acidic phosphoprotein 1; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; ALP, alkaline phosphatase. (G) Mineralized nodule formation was analyzed on days 7 and 14 via Alizarin Red S staining in the Alkbh5 overexpression groups and the control group undergoing odontoblast induction. Scale bars: 2 mm and 100 μm (high magnification). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35784864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DSPP Antibody - Azide and BSA Free [NBP2-92546] -

Alkbh5 was upregulated during mouse odontoblast differentiation in vivo and in vitro. (A) Immunohistochemistry analysis of the expression of ALKBH5 in the PN2.5 murine mandibular incisors. ALKBH5 protein expression was low in (B) pOd and was strongly expressed in (C) polOd (D) sOd, and (E) mOd. (F) The odontoblast markers Dspp and Dmp1, and m6A methylase Mettl3 and demethylase Alkbh5 were upregulated after induction. (G) The protein expression levels of METTL3, ALKBH5, DSPP, and DMP1 were significantly increased. (H) Quantification of the DMP1, DSPP, METTL3, and ALKBH5 protein expression level during odontoblast induction. (A–E) Scale bar: 100 μm Alkbh5, AlkB homolog H5; pOd, pre-odontoblasts; polOd, polarizing odontoblasts; sOd, secretory odontoblasts; mOd, mature odontoblasts; Dspp, dentin sialophosphoprotein; Dmp1, dentin matrix acidic phosphoprotein 1; Mettl3, methyltransferase-like 3; PN2.5, postnatal day 2.5. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35784864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

DSPP Antibody - BSA Free [NBP2-92546] -

Analysis of various lysates using DSPP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.