dTAG-13
Chemical Name: 1-[(2S)-1-Oxo-2-(3,4,5-trimethoxyphenyl)butyl]-(2S)-2-piperidinecarboxylate (1R)-3-(3,4-dimethoxyphenyl)-1-[2-[2-[[6-[[2-(2,6-dioxo-3-piperidinyl)-2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl]oxy]hexyl]amino]-2-oxoethoxy]phenyl]propyl ester
Purity: ≥98%
Biological Activity
dTAG-13 is a degrader targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. Application of dTAG-13 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation.Negative control (Cat. No. 6916) also available.
FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in. Custom knock-in cell lines for the dTAG and aTAG platforms are available from our sister brand R&D Systems. Email TPD@bio-techne.com to enquire.
Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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Additional Information
Background References
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The dTAG system for immediate and target-specific protein degradation.
Nabet et al.
Nat.Chem.Biol., 2018;14:431 -
Transcription control by the ENL YEATS domain in acute leukaemia.
Erb et al.
Nature., 2017;543:270 -
Targeted degradation of SLC transporters reveals amenability of multi-pass transmembrane proteins to ligand-induced proteolysis.
Bensimon et al.
Cell Chem.Biol., 2020;27
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Citations for dTAG-13
The citations listed below are publications that use Tocris products. Selected citations for dTAG-13 include:
5 Citations: Showing 1 - 5
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Engineered PROTAC-CID systems for mammalian inducible gene regulation
Authors: Ma Et al.
J Am Chem Soc 2023;
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Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation.
Authors: Orth-He Et al.
Cell Rep 2023;
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CRASH-IT switch enables reversible and dose-dependent control of TCR and CAR T-cell function.
Authors: Sahillioglu Et al.
Cancer Immunol.Res. 2021;9:999
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Characterization of cereblon-dependent targeted protein degrader by visualizing the spatiotemporal ternary complex formation in cells
Authors: Kaji Et al.
Sci.Rep. 2020;10:3088
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