dTAG-13

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6605/5
dTAG-13 | CAS No. 2064175-41-1 | Active Degraders
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Description: Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation

Chemical Name: 1-[(2S)-1-Oxo-2-(3,4,5-trimethoxyphenyl)butyl]-(2S)-2-piperidinecarboxylate (1R)-3-(3,4-dimethoxyphenyl)-1-[2-[2-[[6-[[2-(2,6-dioxo-3-piperidinyl)-2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl]oxy]hexyl]amino]-2-oxoethoxy]phenyl]propyl ester

Purity: ≥98%

Product Details
Citations (1)
Reviews (1)

Biological Activity

PROTAC targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques (via transgene expression or CRISPR-mediated locus-specific knock-in). Application of dTAG-13 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation.

Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.

Technical Data

M.Wt:
1049.18
Formula:
C57H68N4O15
Solubility:
Soluble to 50 mM in DMSO and to 20 mM in ethanol
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
2064175-41-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from Dana-Farber Cancer Institute

Background References

  1. The dTAG system for immediate and target-specific protein degradation.
    Nabet et al.
    Nat.Chem.Biol., 2018;14:431
  2. Transcription control by the ENL YEATS domain in acute leukaemia.
    Erb et al.
    Nature., 2017;543:270

Product Datasheets

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Citation for dTAG-13

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Works better than expected
By Abderhman Abuhashem on 10/14/2019
Species: Mouse

Used dTAG-13 to degrade a transgene tagged with FKBM12-F36V and HA tags to visualize the protein.


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