dTAG-47

Tocris Bioscience | Catalog # 7530

Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
Tocris Bioscience

Key Product Details

Description

Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation
View all Active Degraders »

Product Description

dTAG-47 is a degrader targeting mutant FKBP12F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand. Application of dTAG-47 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo. dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation. See also dTAG-13 and dTAG-7.

Negative control (Cat. No. 7531) also available.

FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in.

Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.

Licensing Information

Sold under license from Dana-Farber Cancer Institute

Product Specifications for dTAG-47

Molecular Weight

1076.25

Formula

C59H73N5O14

Storage

Store at -20°C

Purity

≥97% (HPLC)

Chemical Name

(1R)-3-(3,4-Dimethoxyphenyl)-1-(2-(2-((8-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-5-yl)amino)octyl)amino)-2-oxoethoxy)phenyl)propyl (2S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate

CAS Number

2265886-81-3

PubChem ID

153280948

InChI Key

OQXAQHQPOQLCAE-ODPQSFPTSA-N

SMILES

CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NCCCCCCCCNC4=CC=C5C(N(C(C5=C4)=O)C6CCC(NC6=O)=O)=O)=O)CCC7=CC(OC)=C(C=C7)OC)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 107.62 100

Preparing Stock Solutions for dTAG-47

The following data is based on the product molecular weight 1076.25.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.93 mL 4.65 mL 9.29 mL
5 mM 0.19 mL 0.93 mL 1.86 mL
10 mM 0.09 mL 0.46 mL 0.93 mL
50 mM 0.02 mL 0.09 mL 0.19 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

Product Documents for dTAG-47

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for dTAG-47

For research use only

Customer Reviews for dTAG-47

There are currently no reviews for this product. Be the first to review dTAG-47 and earn rewards!

Have you used dTAG-47?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card