E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free
Novus Biologicals | Catalog # NB110-85473
![Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]](https://resources.rndsystems.com/images/products/E-selectin-Antibody-RM0034-9J12-Immunohistochemistry-Paraffin-NB110-85473-img0006.jpg "Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]")
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2 Clone # RM0034-9J12
Format
Azide and BSA Free
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Product Specifications
Immunogen
Cytokine-stimulated mouse E-Selectin/CD62E
Clonality
Monoclonal
Host
Rat
Isotype
IgG2
Scientific Data Images for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free
Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]
Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.![Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]](https://resources.rndsystems.com/images/products/E-selectin-Antibody-RM0034-9J12-Immunohistochemistry-Paraffin-NB110-85473-img0005.jpg "Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]")
Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free [NB110-85473]
Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - The Bouins solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to IHC with anti mouse E-Selectin using NB110-85473.Applications for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100 - 1:1000
Immunohistochemistry-Paraffin
1:100 - 1:400
Application Notes
Antigen retrieval: use 20ug/ml proteinase K in PBS for 15-25 mins
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Calculators
Background: E-Selectin/CD62E
Additional E-Selectin/CD62E Products
Product Documents for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free
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Q: This E-selectin antibody is reactive mouse, correct? I have had problems with anti-mouse E-selectin antibodies previously, as many of the commercially available antibodies have not given me the desired vessel staining in IHC. I would like to test this antibody because the image show provided shows good vessel staining.
A:
Yes, this antibody is reactive with mouse. We guarantee all of our products 100% in the applications and species listed on the product datasheet. As this product is recommended for IHC-P and mouse, I think it is a good choice for you. If you are unable to get this product working in IHC-P and mouse on tissues that express the protein, then Novus would be happy to provide a full refund, or send a free of charge replacement product of equal value. View more information about the Novus 100% guarantee.
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