Human EAAT3 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82478

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human EAAT3 in samples. No Significant cross-reactivity or interference between Human EAAT3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.06

Inter-Assay Precision (Precision between assays) CV% 4.97

Recovery for Human EAAT3 ELISA Kit (Colorimetric)

Recovery

86-111%

Linearity

Human EAAT3 ELISA Kit (Colorimetric)

Scientific Data Images for Human EAAT3 ELISA Kit (Colorimetric)

Human EAAT3 ELISA Kit (Colorimetric)

ELISA: Human EAAT3 ELISA Kit (Colorimetric) [NBP2-82478] -

ELISA: Human EAAT3 ELISA Kit (Colorimetric) [NBP2-82478] - Standard Curve Reference

Kit Contents for Human EAAT3 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: EAAT3

The EAAT3 gene encodes for glutamate transporters (also known as excitatory amino acid transporters, EAATs), that rapidly move glutamate from the postsynaptic cleft through the plasma membrane. The proteins are approximately 542 amino acids long at a mass barely over 57 kDA. This function allows for neurotoxic levels of glutamate in the brain to be avoided. It is common to see underactivity of glutamate receptors in some disorders such as ischemia and traumatic brain injuries and overactivity to be linked to mental illnesses such as schizophrenia. EAAT3 gene is also related to obsessive-compulsive disorder, ganglioglioma, autism spectrum disorders, aminoaciduriam temporal lobe epilepsy, hepatic encephalopathy, neuronitis, anorexia nervosa, and amyotrophic lateral sclerosis. It interacts with KNCN, HCCS, EWSR1, PRKCA, and ARL6IP5 to function in pathways of glutamic acid signaling, transmembrane transport of small molecules, and protein digestion and absorption.

Long Name

Excitatory amino acid transporter 3

Alternate Names

EAAC1, SLC1A1

Gene Symbol

SLC1A1

Additional EAAT3 Products

Product Documents for Human EAAT3 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human EAAT3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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