EBAG9/RCAS1 Antibody (4A10) - Azide and BSA Free

Novus Biologicals | Catalog # H00009166-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A10

Format

Azide and BSA Free
View all EBAG9/RCAS1 Primary Antibodies »

Product Specifications

Immunogen

EBAG9 (AAH17729, 1 a.a. ~ 213 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS

Specificity

EBAG9 - estrogen receptor binding site associated, antigen, 9 (4A10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for EBAG9/RCAS1 Antibody (4A10) - Azide and BSA Free

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04]

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04]

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Analysis of EBAG9 expression in HepG2.
Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04]

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04]

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - EBAG9 monoclonal antibody (M04), clone 4A10. Western Blot analysis of EBAG9 expression in MCF-7 ( Cat # L046V1 ).
Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04]

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04]

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Analysis of EBAG9 expression in transfected 293T cell line by EBAG9 monoclonal antibody (M04), clone 4A10. Lane 1: EBAG9 transfected lysate (Predicted MW: 23.54 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Detection limit for recombinant GST tagged EBAG9 is 0.1 ng/ml as a capture antibody.

Applications for EBAG9/RCAS1 Antibody (4A10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EBAG9/RCAS1

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]

Alternate Names

Cancer-associated surface antigen RCAS1, EB9cancer associated surface antigen, estrogen receptor binding site associated, antigen, 9, Estrogen receptor-binding fragment-associated gene 9 protein, RCAS1PDAF, receptor-binding cancer antigen expressed on SiSo cells

Entrez Gene IDs

9166 (Human)

Gene Symbol

EBAG9

UniProt

AAH17729 (Human)

Additional EBAG9/RCAS1 Products

Product Documents for EBAG9/RCAS1 Antibody (4A10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EBAG9/RCAS1 Antibody (4A10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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