eEF1A1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-32122
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Golden Syrian Hamster
Predicted:
Bovine (100%), Canine (100%), Chicken (99%), Feline (100%), Porcine (100%), Primate (100%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (98%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human eEF1A1. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (100%), Cat (100%), Xenopus laevis (98%).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for eEF1A1 Antibody - BSA Free
Western Blot: eEF1A1 Antibody [NBP1-32122]
Western Blot: eEF1A1 Antibody [NBP1-32122] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Western Blot: eEF1A1 Antibody [NBP1-32122]
Western Blot: eEF1A1 Antibody [NBP1-32122] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Western Blot: eEF1A1 Antibody [NBP1-32122]
Western Blot: eEF1A1 Antibody [NBP1-32122] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Western Blot: eEF1A1 Antibody [NBP1-32122]
Western Blot: eEF1A1 Antibody [NBP1-32122] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eEF1A1 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for eEF1A1 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: eEF1A1
Alternate Names
CCS3, CCS-3, cervical cancer suppressor 3, CTCL tumor antigen, EE1A1, EEF-1, EEF1A, eEF1A-1, EF1AHNGC:16303, EF1a-like protein, EF-1-alpha-1, EF-Tu, elongation factor 1 alpha subunit, elongation factor 1-alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, eukaryotic translation elongation factor 1 alpha 1, eukaryotic translation elongation factor 1 alpha 1-like 14, FLJ25721, glucocorticoid receptor AF-1 specific elongation factor, GRAF-1EF, LENG7, leukocyte receptor cluster (LRC) member 7, Leukocyte receptor cluster member 7, MGC102687, MGC131894, MGC16224, prostate tumor-inducing protein 1, PTI1, translation elongation factor 1 alpha 1-like 14
Entrez Gene IDs
1915 (Human)
Gene Symbol
EEF1A1
UniProt
Additional eEF1A1 Products
Product Documents for eEF1A1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for eEF1A1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for eEF1A1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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