EEF2K [p Ser78] Antibody

Novus Biologicals | Catalog # NBP3-23304

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all EEF2K Primary Antibodies »

Product Specifications

Immunogen

Phospho-eEF2K (Ser-78) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-78 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins. [UniProt# O00418]

Modification

p Ser78

Specificity

This antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of mouse C2C12 and Jurkat cells treated with Calyculin A, and this reactivity is removed after lambda phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for EEF2K [p Ser78] Antibody

EEF2K [p Ser78] Antibody

Western Blot: EEF2K [p Ser78] Antibody [NBP3-23304] -

Western Blot: EEF2K [p Ser78] Antibody [NBP3-23304] - Western blot image of C2C12 treated with calyculin A (100 nM) for 30 min. (lanes 1 & 3), then the blot was treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-EEF2K (N-terminus) at 1:500 (lanes 1 & 2) and anti-EEF2K (Ser-78) (NBP3-23304) at 1:1000.

Applications for EEF2K [p Ser78] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: EEF2K

EEF2K encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

Alternate Names

Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase, EC 2.7.11, EC 2.7.11.20, eEF-2 kinase, eEF-2Kcalmodulin-dependent protein kinase III, elongation factor-2 kinase, eukaroytic elongation factor 2 kinase, eukaryotic elongation factor 2 kinase, eukaryotic elongation factor-2 kinase, HSU93850, MGC45041

Gene Symbol

EEF2K

Additional EEF2K Products

Product Documents for EEF2K [p Ser78] Antibody

Certificate of Analysis

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Product Specific Notices for EEF2K [p Ser78] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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