EGF Antibody (R001) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89581

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # R001 expressed in HEK293

Format

Azide and BSA Free
View all EGF Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human EGF (Accession#: NP_001954.2; Asn971-Arg1023)

Specificity

Has cross-reactivity in ELISA with: Canine EGFMouse EGF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EGF Antibody (R001) - Azide and BSA Free

Neutralization: EGF Antibody (R001) [NBP2-89581]

Neutralization: EGF Antibody (R001) [NBP2-89581]

Neutralization: EGF Antibody (R001) [NBP2-89581] - Recombinant Human EGF stimulates proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized by increasing concentrations of Human EGF Monoclonal Antibody. The IC50 is typically 2.5-7.5 ug/mL.

Applications for EGF Antibody (R001) - Azide and BSA Free

Application
Recommended Usage

Neutralization

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in Histidine and Arginine buffer, 120mM NaCl, 0.02% Tween 80, pH6.0

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGF

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Gene Symbol

EGF

Additional EGF Products

Product Documents for EGF Antibody (R001) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EGF Antibody (R001) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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