EGLN2/PHD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92974

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EGLN2/PHD1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human EGLN2/PHD1 (NP_444274.1). EVEALKRGGRLRDGQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EGLN2/PHD1 Antibody - BSA Free

Western Blot: EGLN2/PHD1 AntibodyBSA Free [NBP2-92974]

Western Blot: EGLN2/PHD1 AntibodyBSA Free [NBP2-92974]

Western Blot: EGLN2/PHD1 Antibody [NBP2-92974] - Analysis of extracts of various cell lines, using EGLN2/PHD1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
EGLN2/PHD1 Antibody - BSA Free

Immunoprecipitation: EGLN2/PHD1 Antibody - BSA Free [NBP2-92974] -

Immunoprecipitation: EGLN2/PHD1 Antibody - BSA Free [NBP2-92974] - Immunoprecipitation analysis of 150 ug extracts of HeLa cells using 3 ug Prolyl hydroxylase PHD1 (EGLN2/PHD1) antibody. Western blot was performed from the immunoprecipitate using Prolyl hydroxylase PHD1 (EGLN2/PHD1) antibody at a dilution of 1:1000.

Applications for EGLN2/PHD1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EGLN2/PHD1

HIF prolyl hydroxylase 1 (HPH-3 or EGLN2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.

Long Name

Egl Nine Homolog 2/Prolyl Hydroxylase Domain-containing Protein 1

Alternate Names

EIT6, HIFPH1, HPH-1, HPH-3, PHD1

Gene Symbol

EGLN2

Additional EGLN2/PHD1 Products

Product Documents for EGLN2/PHD1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EGLN2/PHD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EGLN2/PHD1 Antibody - BSA Free

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