EGR1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-56101

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EGR1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: IFPEPQSQAFPGSAGTALQYPPPAYPAAKGGFQVPMIPDYLFPQQQGDLGLGTPDQKPFQGLESRTQQPSLTPLSTIKAFATQSGSQDLKALNTSYQSQLI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EGR1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EGR1 Antibody [NBP2-56101]

Immunocytochemistry/ Immunofluorescence: EGR1 Antibody [NBP2-56101]

Immunocytochemistry/Immunofluorescence: EGR1 Antibody [NBP2-56101] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
EGR1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: EGR1 Antibody - BSA Free [NBP2-56101]

Chromatin Immunoprecipitation-exo-Seq: EGR1 Antibody - BSA Free [NBP2-56101]

ChIP-Exo-Seq composite graph for Anti-EGR1 (NBP2-56101) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for EGR1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGR1

Egr1 is encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq]

Long Name

Early Growth Response 1

Alternate Names

KROX24, NGFIA, ZNF225

Gene Symbol

EGR1

Additional EGR1 Products

Product Documents for EGR1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EGR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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