EIF3S3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03812

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EIF3S3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3S3 (NP_003747.1). NCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWYQSTYYGSFVTRALLDSQFSYQHAIEESVVLIYDPIKTAQGSLSLKAYRLTPKLMEVCKEKDFSPEALKKANITFEYMFEEVPIVIKNSHLINVLMWELEKKSAVADKHELLSLASSNHLGKNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEEDLSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EIF3S3 Antibody - BSA Free

Western Blot: EIF3S3 AntibodyBSA Free [NBP3-03812]

Western Blot: EIF3S3 AntibodyBSA Free [NBP3-03812]

Western Blot: EIF3S3 Antibody [NBP3-03812] - Analysis of extracts of various cell lines, using EIF3S3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
EIF3S3 Antibody - BSA Free

Immunoprecipitation: EIF3S3 Antibody - BSA Free [EIF3S3] -

Immunoprecipitation: EIF3S3 Antibody - BSA Free [EIF3S3] - Immunoprecipitation analysis of 200 ug extracts of Jurkat cells using 1 ug EIF3S3 antibody. Western blot was performed from the immunoprecipitate using EIF3S3 antibody at a dilution of 1:1000.
EIF3S3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EIF3S3 Antibody - BSA Free [NBP3-03812] -

Immunocytochemistry/ Immunofluorescence: EIF3S3 Antibody - BSA Free [NBP3-03812] - Immunofluorescence analysis of U2OS cells using EIF3S3 Rabbit pAb (A13378). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for EIF3S3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EIF3S3

Eukaryotic initiation factor 3 subunit H (eIF3H) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3H is a non-conserved subunit and part of the functional core of eIF3. It may function to stabilize the eIF3 complex and stimulate protein synthesis. Many cancer cell lines have been shown to express high levels of eIF3H, and it may have an oncogenic role in colorectal cancer susceptibility.

Alternate Names

eIF-3-gamma, eIF3-gamma, eIF3h, eIF3-p40, EIF3S3eIF3 p40 subunit, Eukaryotic translation initiation factor 3 subunit 3, eukaryotic translation initiation factor 3 subunit H, eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD), eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD), eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa, eukaryotic translation initiation factor 3, subunit H, MGC102958

Gene Symbol

EIF3H

Additional EIF3S3 Products

Product Documents for EIF3S3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EIF3S3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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