eIF4A2 Antibody (PSH01-89)

Novus Biologicals | Catalog # NBP3-32301

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH01-89 expressed in HEK293
View all eIF4A2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human eIF4A2 aa 1-407 / 407. (Uniprot: Q14240)

Localization

Cytosol.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4A2 Antibody (PSH01-89)

eIF4A2 Antibody (PSH01-89) Western Blot: eIF4A2 Antibody (PSH01-89) [NBP3-32301]

Western Blot: eIF4A2 Antibody (PSH01-89) [NBP3-32301]

Western blot analysis on different lysates with NBP3-32301 at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-3M cell lysate Lane 4: 293T cell lysate Lane 5: MCF7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C2C12 cell lysate Lane 8: PC-12 cell lysate Lane 9: COS-1 cell lysate Lane 10: Human brain tissue lysate Lane 11: Mouse testis tissue lysate Lane 12: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32301 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for eIF4A2 Antibody (PSH01-89)

Application
Recommended Usage

Immunoprecipitation

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF4A2

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Alternate Names

ATP-dependent RNA helicase eIF4A-2, BM-010, DDX2Beukaryotic translation initiation factor 4A, isoform 2, EC 3.6.1, EC 3.6.4.13, EIF4A, eIF-4A-II, eIF4A-II, EIF4F, eukaryotic initiation factor 4A-II, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A2

Gene Symbol

EIF4A2

Additional eIF4A2 Products

Product Documents for eIF4A2 Antibody (PSH01-89)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for eIF4A2 Antibody (PSH01-89)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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