eIF5A Antibody (3R10I7)

Novus Biologicals | Catalog # NBP3-16409

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3R10I7 expressed in HEK293
View all eIF5A Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF5A (P63241). MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF5A Antibody (3R10I7)

Western Blot: eIF5A Antibody (3R10I7) [NBP3-16409]

Western Blot: eIF5A Antibody (3R10I7) [NBP3-16409]

Western Blot: eIF5A Antibody (3R10I7) [NBP3-16409] - Western blot analysis of extracts of various cell lines, using eIF5A Rabbit mAb (NBP3-16409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for eIF5A Antibody (3R10I7)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF5A

The human eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein. eIF5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post translational reactions. eIF5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.

Long Name

Eukaryotic Translation Initiation Factor 5A

Alternate Names

eIF4D, Rev-binding Factor

Gene Symbol

EIF5A

Additional eIF5A Products

Product Documents for eIF5A Antibody (3R10I7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for eIF5A Antibody (3R10I7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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