Elastase Antibody (CELA3B/1758) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08863

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # CELA3B/1758

Format

Azide and BSA Free
View all Elastase Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Elastase protein fragment (aa82-238) (exact sequence is proprietary) (Uniprot: P08861)

Localization

Cytoplasmic

Marker

Pancreatic Function Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07538).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for Elastase Antibody (CELA3B/1758) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml
Application Notes
ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA. Western Blot 1-2ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Elastase

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have sixelastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike otherelastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted fromthe pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has adigestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3Bmay also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B havebeen referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently usedas a measure of pancreatic function in clinical assays. (provided by RefSeq)

Alternate Names

CBPP, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, chymotrypsin-like elastase family, member 3B, E1, EC 3.4.21, EC 3.4.21.70, EL-1, ELA3B, elastase 1, elastase 3B, pancreatic, Elastase IIIB, Elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, Protease E, proteinase E

Gene Symbol

CELA3B

Additional Elastase Products

Product Documents for Elastase Antibody (CELA3B/1758) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Elastase Antibody (CELA3B/1758) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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