ELF3/ESE-1 Antibody (7O2V4)

Novus Biologicals | Catalog # NBP3-16429

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7O2V4 expressed in HEK293
View all ELF3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-371 of human ELF3/ESE-1 (P78545). MAATCEISNIFSNYFSAMYSSEDSTLASVPPAATFGADDLVLTLSNPQMSLEGTEKASWLGEQPQFWSKTQVLDWISYQVEKNKYDASAIDFSRCDMDGATLCNCALEELRLVFGPLGDQLHAQLRDLTSSSSDELSWIIELLEKDGMAFQEALDPGPFDQGSPFAQELLDDGQQASPYHPGSCGAGAPSPGSSDVSTAGTGASRSSHSSDSGGSDVDLDPTDGKLFPSDGFRDCKKGDPKHGKRKRGRPRKLSKEYWDCLEGKKSKHAPRGTHLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRRLVYKFGKNSSGWKEEEVLQSRN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ELF3/ESE-1 Antibody (7O2V4)

Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429]

Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429]

Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Western blot analysis of extracts of various cell lines, using ELF3/ESE-1 Rabbit mAb (NBP3-16429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
ELF3/ESE-1 Antibody (7O2V4)

Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] -

Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Analysis of extracts of HepG2 cells, using ESE1/ELF3 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ELF3/ESE-1 Antibody (7O2V4)

Chip IP Sequencing: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] -

Chip IP Sequencing: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] -CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ HepG5 with 1μg ESE1 along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of ESE1 in representative gene loci (MARK4), as shown in figure.
ELF3/ESE-1 Antibody (7O2V4)

Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] -

Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using ELF3/ESE-1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ELF3/ESE-1 Antibody (7O2V4)

Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [ELF3/ESE-1] -

Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [ELF3/ESE-1] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using ELF3/ESE-1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for ELF3/ESE-1 Antibody (7O2V4)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5μg antibody for 10μg-15μg of Chromatin

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Western Blot

1:1000 - 1:6000
Application Notes
CUT&Tag, 10 cells /1 ug

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ELF3

ESE-1 (Epithelium-Specific Ets) is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, which has features distinct from those of any other ets-related factor. ESE-1 binds with high affinity to and transactivates the ets binding site in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A. ESE-1 is a critical regulator of epithelial cell differentiation.

Long Name

E74-like factor 3

Alternate Names

EPR1, ERT, ESX, JEN

Gene Symbol

ELF3

Additional ELF3 Products

Product Documents for ELF3/ESE-1 Antibody (7O2V4)

Certificate of Analysis

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Product Specific Notices for ELF3/ESE-1 Antibody (7O2V4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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