ELYS Antibody - BSA Free

Novus Biologicals | Catalog # NB600-238

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation

Cited:

Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A syntetic peptide between aa2220 and C-term

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ELYS Antibody - BSA Free

Immunoprecipitation: ELYS Antibody [NB600-238]

Immunoprecipitation: ELYS Antibody [NB600-238]

Immunoprecipitation: ELYS Antibody [NB600-238] - Nuclear Extract (10 and 20 ug for WB; 1 mg for IP/WB) from HeLa cells. Antibodies: A) Affinity purified rabbit anti-ELYS antibody BL476 used at 0.5 ug/ml for WB and 4 ug/mg lysate for IP. B) Affinity purified rabbit anti-ELYS antibodies BL476 and BL475 were each used at 4 ug/mg extract for IP. Immunoprecipitated ELYS was detected by WB using BL475 at 0.2 ug/ml. Detection: Chemiluminescence with exposure times of 3 to 5 minutes.

Applications for ELYS Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in Simple Western reported in scientific literature (PMID:35017527) By Western Blot, this antibody recognizes a band at ~252.5 kDa, representing ELYS protein. May be used for immunoprecipitation, as well. Western blot of immunoprecipitates was performed using an immunoprecipitation/Western blot reagent and 4-8% SDS-PAGE.
See Simple Western Antibody Database for Simple Western validation

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ELYS

ELYS is a novel nuclear transcription factor expressed in embryonic haematopoietic tissues and plays a role in both early and mature haematopoietic events.

Alternate Names

AT hook containing transcription factor 1, ELYS, ELYS transcription factor like protein TMBS62, ELYS transcription factor-like protein TMBS62, MST108, MSTP108, protein ELYS, putative AT-hook-containing transcription factor 1, TMBS62

Entrez Gene IDs

25909 (Human)

Gene Symbol

AHCTF1

Additional ELYS Products

Product Documents for ELYS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ELYS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ELYS Antibody - BSA Free

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