ELYS Antibody - BSA Free
Novus Biologicals | Catalog # NB600-238
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Simple Western, Immunoprecipitation
Cited:
Simple Western, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A syntetic peptide between aa2220 and C-term
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ELYS Antibody - BSA Free
Immunoprecipitation: ELYS Antibody [NB600-238]
Immunoprecipitation: ELYS Antibody [NB600-238] - Nuclear Extract (10 and 20 ug for WB; 1 mg for IP/WB) from HeLa cells. Antibodies: A) Affinity purified rabbit anti-ELYS antibody BL476 used at 0.5 ug/ml for WB and 4 ug/mg lysate for IP. B) Affinity purified rabbit anti-ELYS antibodies BL476 and BL475 were each used at 4 ug/mg extract for IP. Immunoprecipitated ELYS was detected by WB using BL475 at 0.2 ug/ml. Detection: Chemiluminescence with exposure times of 3 to 5 minutes.Applications for ELYS Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in Simple Western reported in scientific literature (PMID:35017527) By Western Blot, this antibody recognizes a band at ~252.5 kDa, representing ELYS protein. May be used for immunoprecipitation, as well. Western blot of immunoprecipitates was performed using an immunoprecipitation/Western blot reagent and 4-8% SDS-PAGE.
See Simple Western Antibody Database for Simple Western validation
See Simple Western Antibody Database for Simple Western validation
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ELYS
Alternate Names
AT hook containing transcription factor 1, ELYS, ELYS transcription factor like protein TMBS62, ELYS transcription factor-like protein TMBS62, MST108, MSTP108, protein ELYS, putative AT-hook-containing transcription factor 1, TMBS62
Entrez Gene IDs
25909 (Human)
Gene Symbol
AHCTF1
Additional ELYS Products
Product Documents for ELYS Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for ELYS Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for ELYS Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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