Emerin Antibody (EMD/2168) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08893

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # EMD/2168

Format

Azide and BSA Free
View all Emerin Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Emerin protein fragment (around aa 56-167) (exact sequence is proprietary) (Uniprot: P50402)

Localization

Nuclear Membrane

Marker

Papillary Thyroid Carcinoma and EDMD Marker

Specificity

Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07151).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Emerin Antibody (EMD/2168) - Azide and BSA Free

Western Blot: Emerin Antibody (EMD/2168)Azide and BSA Free [NBP3-08893]

Western Blot: Emerin Antibody (EMD/2168)Azide and BSA Free [NBP3-08893]

Western Blot: Emerin Antibody (EMD/2168) [NBP3-08893] - Western Blot Analysis of human HeLa cell lysate using Emerin Mouse Monoclonal Antibody (EMD/2168).
Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2168) - Azide and BSA Free [NBP3-08893]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2168) - Azide and BSA Free [NBP3-08893]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2168) [NBP3-08893] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2168).
Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2168) - Azide and BSA Free [NBP3-08893]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2168) - Azide and BSA Free [NBP3-08893]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2168) [NBP3-08893] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2168).
Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2168) - Azide and BSA Free [NBP3-08893]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2168) - Azide and BSA Free [NBP3-08893]

Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2168) [NBP3-08893] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2168).
Protein Array: Emerin Antibody (EMD/2168) - Azide and BSA Free [NBP3-08893]

Protein Array: Emerin Antibody (EMD/2168) - Azide and BSA Free [NBP3-08893]

Protein Array: Emerin Antibody (EMD/2168) [NBP3-08893] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Mouse Emerin Monoclonal Antibody (EMD/2168).

Applications for Emerin Antibody (EMD/2168) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Emerin

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Alternate Names

emerin, Emery-Dreifuss muscular dystrophy, LEM domain containing 5, STAEDMDLEMD5

Gene Symbol

EMD

Additional Emerin Products

Product Documents for Emerin Antibody (EMD/2168) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Emerin Antibody (EMD/2168) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Emerin Antibody (EMD/2168) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Emerin Antibody (EMD/2168) - Azide and BSA Free and earn rewards!

Have you used Emerin Antibody (EMD/2168) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies