EML4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86805

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EML4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSIIQWKLVEKLSLPQNETVADTTLTKAPVSSTESVIQSNTPTPPPSQPLNETAEEESRISSSPTLLENSLEQTVEPSEDHSEEES

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

109 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EML4 Antibody - BSA Free

EML4 Antibody - BSA Free Immunohistochemistry-Paraffin: EML4 Antibody - BSA Free [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody - BSA Free [NBP1-86805]

Staining of human cell line U-251 MG shows localization to cytosol, intermediate filaments & microtubules.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex, gallbladder, kidney and lymph node using Anti-EML4 antibody NBP1-86805 (A) shows similar protein distribution across tissues to independent antibody NBP2-38319 (B).
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human lymph node.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human kidney.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human gallbladder.
EML4 Antibody - BSA Free Western Blot: EML4 Antibody - BSA Free [NBP1-86805]

Western Blot: EML4 Antibody - BSA Free [NBP1-86805]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4

Applications for EML4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EML4

Echinoderm microtubule associated protein like 4 (EML4) has been identified as part of a gene fusion with the kinase domain of the anaplastic lymphoma receptor tyrosine kinase (ALK) in non-small cell lung cancer. EML4 associates with in vitro polymerized microtubules. In the cell, phosphorylated EML4 associates with the mitotic spindle and is essential for proliferation and microtubule network formation. Alternative names for EML4 include EMAPL4, ropp120, C2orf2, ELP120, and EMAP-4.

Alternate Names

C2orf2, echinoderm microtubule associated protein like 4, echinoderm microtubule-associated protein-like 4, ELP120, EMAP-4, EMAPL4, FLJ10942, FLJ32318, Restrictedly overexpressed proliferation-associated protein, Ropp 120, ROPP120DKFZp686P18118

Entrez Gene IDs

27436 (Human)

Gene Symbol

EML4

UniProt

Q9HC35 (Human)

Additional EML4 Products

Product Documents for EML4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EML4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EML4 Antibody - BSA Free

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Protocols

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