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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic 18 amino acid peptide from C-terminus of human EMR1.
Epitope
C-Terminus
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Gorilla (89%), Marmoset (89%), Gibbon (83%).
Specificity
Human EMR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Product can be stored undiluted at 4C for up to 1 month.
Scientific Data Images for EMR1 Antibody - BSA Free
Immunohistochemistry-Paraffin: EMR1 Antibody - BSA Free [NLS2678]
Immunohistochemistry-Paraffin: EMR1 Antibody [NLS2678] - Anti-EMR1 / F4/80 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.![Immunohistochemistry-Paraffin: EMR1 Antibody - BSA Free [NLS2678]](https://resources.rndsystems.com/images/products/EMR1-Antibody-Immunohistochemistry-Paraffin-NLS2678-img0001.jpg "Immunohistochemistry-Paraffin: EMR1 Antibody - BSA Free [NLS2678]")
Immunohistochemistry-Paraffin: EMR1 Antibody - BSA Free [NLS2678]
Immunohistochemistry-Paraffin: EMR1 Antibody [NLS2678] - Analysis of anti-EMR1 / F4/80 antibody with bone marrow at 36 ug/ ml.![Immunohistochemistry-Paraffin: EMR1 Antibody - BSA Free [NLS2678]](https://resources.rndsystems.com/images/products/EMR1-Antibody-Immunohistochemistry-Paraffin-NLS2678-img0002.jpg "Immunohistochemistry-Paraffin: EMR1 Antibody - BSA Free [NLS2678]")
Immunohistochemistry-Paraffin: EMR1 Antibody - BSA Free [NLS2678]
Immunohistochemistry-Paraffin: EMR1 Antibody [NLS2678] - Analysis of anti-EMR1 / F4/80 antibody with human breast, carcinoma.
Immunohistochemistry-Paraffin: EMR1 Antibody - BSA Free [NLS2678] -
Immunohistochemistry-Paraffin: EMR1 Antibody - BSA Free [NLS2678] - Immunohistochemistry of formalin-fixed, paraffin-embedded human Testis tissue after heat-induced antigen retrieval.Applications for EMR1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
10-15 ug/ml
Application Notes
.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: EMR1
Long Name
EGF-like Module Containing, Mucin-like, Hormone Receptor-like 1
Alternate Names
ADGRE1, TM7LN3
Entrez Gene IDs
2015 (Human)
Gene Symbol
ADGRE1
Additional EMR1 Products
Product Documents for EMR1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EMR1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for EMR1 Antibody - BSA Free
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Q: I have noticed that two of your F4/80 (EMR1) antibodies (NLS2675 and NLS2678) are listed as reactive with the human protein. I am curious as to whether you have tested these reagents against the mouse protein as well, or whether you have a prediction of reactivity based on homology.
A: We have not actually tested this antibody with mouse but the homology with mouse is only 65%. This is not something I would not recommend to test as our lab only recommends use if the homology is 85% or above.
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