ENO3 Antibody (S07-5B2)

Novus Biologicals | Catalog # NBP3-14890

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-5B2 expressed in HEK293
View all ENO3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human ENO3 (Uniprot#: P13929)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ENO3 Antibody (S07-5B2)

Western Blot: ENO3 Antibody (S07-5B2) [NBP3-14890]

Western Blot: ENO3 Antibody (S07-5B2) [NBP3-14890]

Western Blot: ENO3 Antibody (S07-5B2) [NBP3-14890] - Western blot detection of ENO3 in Rat Brain, C6, 3T3, Hela cell lysates using ENO3 Rabbit mAb (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.
ENO3 Antibody (S07-5B2)

Immunocytochemistry/Immunofluorescence: ENO3 Antibody (S07-5B2) [NBP3-14890] -

Immunocytochemistry/Immunofluorescence: ENO3 Antibody (S07-5B2) [NBP3-14890] - Immunofluorescence of ENO3 (green) in Hela cells using NBP3-14890 at dilution 1/50, and DAPI (blue)

Applications for ENO3 Antibody (S07-5B2)

Application
Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENO3

ENO3 encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.

Alternate Names

2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, beta-enolase, EC 4.2.1, EC 4.2.1.11, Enolase 3, enolase 3 (beta, muscle), enolase 3, (beta, muscle), GSD13, MSE, Muscle-specific enolase, Skeletal muscle enolase

Gene Symbol

ENO3

Additional ENO3 Products

Product Documents for ENO3 Antibody (S07-5B2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ENO3 Antibody (S07-5B2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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