Recombinant Human Enolase 1 Protein

Novus Biologicals | Catalog # NBP1-30262

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Western Blot, Bioactivity, Microarray, SDS-PAGE
View all Enolase 1 Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-434 of Human Enolase 1

Source: E.coli

Amino Acid Sequence: MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

47.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

Application Notes

Use in Microarray reported in scientific literature (PMID:35059422)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Enolase 1 Protein

Western Blot: Recombinant Human Enolase 1 Protein [NBP1-30262]

Western Blot: Recombinant Human Enolase 1 Protein [NBP1-30262]

Western Blot: Recombinant Human Enolase 1 Protein [NBP1-30262] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Enolase 1 antibody [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.
SDS-PAGE: Recombinant Human Enolase 1 Protein [NBP1-30262]

SDS-PAGE: Recombinant Human Enolase 1 Protein [NBP1-30262]

SDS-Page: Recombinant Human Enolase 1 Protein [NBP1-30262] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP1-30262
Formulation 20 mM Tris-HCl buffer (pH 7.5), 1 mM MgSO4, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enolase 1

Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin

Gene Symbol

ENO1

Additional Enolase 1 Products

Product Documents for Recombinant Human Enolase 1 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Enolase 1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human Enolase 1 Protein

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Protocols

View specific protocols for Recombinant Human Enolase 1 Protein (NBP1-30262):

SDS-PAGE (NBP1-30262):
1. Prepare 1,450ul assay buffer. The final concentrations are 81 mM Triethanolamine, 1.9 mM 2-phosphorglycerate, 0.12 mM beta- NADPH, 25 mM magnesium sulfate, 100 mM potassium chloride, 1.3 mM ADP, 4 unit pyruvate kinase, 6 unit L-lactic dehydrogenas.


2. Add 50ul of recombinant protein alpha-enolase in various concentrations(0.25 ug, 0.5 ug, and 0.1 ug) in assay buffer.


3. Mix by inversion and load 200 ul of reaction mix in to a plate well.


4. Record the decrease in A340nm for 5 minutes at 25C.

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