ENPP-2/Autotaxin Antibody

Novus Biologicals | Catalog # NBP1-32162

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ENPP-2/Autotaxin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ENPP-2/Autotaxin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ENPP-2/Autotaxin Antibody

Immunocytochemistry/ Immunofluorescence: ENPP-2/Autotaxin Antibody [NBP1-32162]

Immunocytochemistry/ Immunofluorescence: ENPP-2/Autotaxin Antibody [NBP1-32162]

Immunocytochemistry/Immunofluorescence: ENPP-2/Autotaxin Antibody [NBP1-32162] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Autotaxin protein stained by Autotaxin antibody diluted at 1:500. Blue: Hoechst 33342 staining.
ENPP-2/Autotaxin Antibody

Western Blot: ENPP-2/Autotaxin Antibody [NBP1-32162] -

Western Blot: ENPP-2/Autotaxin Antibody [NBP1-32162] - Human Cerebrospinal Fluid (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ENPP-2/Autotaxin antibody (NBP1-32162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ENPP-2/Autotaxin Antibody

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

Reported in scientific literature (PMID: 28739650)

Western Blot

1:500-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ENPP-2/Autotaxin

The protein encoded by the ENPP2 gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 2

Alternate Names

ATX, Autotaxin, ENPP2, Lysophosphatidic Acid, NPP2, PDNP2

Entrez Gene IDs

5168 (Human); 18606 (Mouse)

Gene Symbol

ENPP2

UniProt

Q13822

Additional ENPP-2/Autotaxin Products

Product Documents for ENPP-2/Autotaxin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ENPP-2/Autotaxin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Phosphatases and Regulators

Citations for ENPP-2/Autotaxin Antibody

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Protocols

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