EphA4 Antibody (M280)

Novus Biologicals | Catalog # NBP3-23328

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M280
View all EphA4 Primary Antibodies »

Product Specifications

Immunogen

Clone M280 was generated from a recombinant protein corresponding to amino acids in the N-terminal region of human EphA4. This region has less than 50% homology to similar regions in other EphA and EphB family members. [UniProt# P54764]

Specificity

This antibody detects 95 and 120 kDa bands corresponding to EphA4 in Western blots of HUVEC cells and a 120 kDa band in mouse brain tissue.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for EphA4 Antibody (M280)

EphA4 Antibody (M280)

Western Blot: EphA4 Antibody (M280) [NBP3-23328] -

Western Blot: EphA4 Antibody (M280) [NBP3-23328] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).

Applications for EphA4 Antibody (M280)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: EphA4

Eph receptor A4 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Long Name

Eph Receptor A4

Alternate Names

Cek8, Hek8, Sek1, Tyro1

Gene Symbol

EPHA4

Additional EphA4 Products

Product Documents for EphA4 Antibody (M280)

Certificate of Analysis

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Product Specific Notices for EphA4 Antibody (M280)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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