Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-31072

Novus Biologicals
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.067 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)
View all epithelial Sodium Channel alpha ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images for Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric)

Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric)

ELISA: Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric) [NBP3-31072] -

ELISA: Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric) [NBP3-31072] - Standard Curve Reference

Kit Contents for Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: epithelial Sodium Channel alpha

Epithelial Sodium Channel alpha (SCNN1A) is a subunit of the epithelial sodium channel (ENaC). ENac has high sodium selectivity, low conductance, and amiloride sensitivity. The functional channel of ENaC is composed of at least 3 subunits, alpha (SCNN1A), beta (SCNN1B), and gamma (SCNN1G). The 3 subunits show sequence similarities to one another, indicating descent from a common ancestral gene. Each encodes a protein containing 2 transmembrane domains, with intracellular amino and carboxyl termini. The alpha subunit supports sodium conductance when expressed alone; the beta and gamma subunits do not support sodium conductance by themselves, but greatly augment the channel activity when expressed in conjunction with the alpha subunit. ENaC in the kidney, lung and colon plays an essential role in trans- epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel's syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).

Long Name

Amiloride-sensitive sodium channel subunit alpha

Alternate Names

Alpha-ENaC, Alpha-NaCH, ENaCA, SCNEA, SCNN1, SCNN1A

Gene Symbol

SCNN1A

Additional epithelial Sodium Channel alpha Products

Product Documents for Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customer Reviews for Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric)

There are currently no reviews for this product. Be the first to review Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric) and earn rewards!

Have you used Human epithelial Sodium Channel alpha ELISA Kit (Colorimetric)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits