Q: I need the names of the primary and secondary antibodies used in an EBV immunohistochemical method.

A: We have the following primary antibodies for EBV that have been validated in IHC. You would then choose a secondary dependent on the host of the primary you choose - anti-rat or anti-mouse - and conjugated to the label of your choice.

Epstein Barr Virus Antibody (CS3) [DyLight 488]

Novus Biologicals | Catalog # NBP3-08631G

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Product Documents

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # CS3

View all Epstein Barr Virus Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing the sequence of bacterial beta-galactosidase and the carboxyl half of EBV-encoded LMP

Reactivity Notes

EBV

Localization

Nuclear

Specificity

This antibody is a mixture of four different monoclonal antibodies. This antibody is specific to 60kDa latent membrane protein (LMP-1) encoded by the BNLF1 gene of the EBV. Each clone reacts with different epitopes on the hydrophilic C-terminus of the cytoplasmic domain of LMP-1. This antibody stains strongly with EBV-positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis. EBV, also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV infects B cells and, though often asymptomatic, it can cause infectious mononucleosis, a disease characterized by fatigue, fever, sore throat and muscle soreness.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Applications for Epstein Barr Virus Antibody (CS3) [DyLight 488]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Epstein Barr Virus

Epstein Barr virus (EBV) is a member of the herpesvirus family and one of the most common human viruses. Most people become infected with EBV during their lives. Primary infections usually results in infectious mononucleosis (glandular fever) but the virus can also lay dormant in B lymphocytes and when reactivated become associated with more serious disease such as Burkitt's lymphoma, nasopharyngeal carcinoma and Hodgkin's disease.

Alternate Names

Epstein Barr Virus, Human herpesvirus type 4, Hypothetical protein BALF1

Additional Epstein Barr Virus Products

Product Documents for Epstein Barr Virus Antibody (CS3) [DyLight 488]

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Certificate of Analysis

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Product Specific Notices for Epstein Barr Virus Antibody (CS3) [DyLight 488]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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