ErbB2/Her2 Antibody [ADC] - (Trastuzumab Vedotin, Research Grade)

Novus Biologicals | Catalog # NBP3-44528

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

ADC

Antibody Source

Monoclonal Human IgG1
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

ErbB2/Her2 (Uniprot: P04626)

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Theoretical MW

150.63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This product is an Antibody-Drug Conjugate (ADC) for Research Use Only (RUO).

Trastuzumab MMAE is an ADC consisting of a HER2-directed antibody and Monomethyl auristatin E (MMAE) used for the study of breast cancer.

This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ErbB2/Her2 Antibody [ADC] - (Trastuzumab Vedotin, Research Grade)

Hu ErbB2/Her2 mAb [ADC] - (Trastuzumab Vedotin)

ELISA: Hu ErbB2/Her2 mAb [ADC] - (Trastuzumab Vedotin) [NBP3-44528]

Immobilized human HER2 His at 2 ug/mL can bind ErbB2/Her2, EC50 is 0.008281 ug/mL.

Applications for ErbB2/Her2 Antibody [ADC] - (Trastuzumab Vedotin, Research Grade)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

> 90% by SDS-PAGE and HPLC

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody [ADC] - (Trastuzumab Vedotin, Research Grade)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ErbB2/Her2 Antibody [ADC] - (Trastuzumab Vedotin, Research Grade)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB2/Her2 Antibody [ADC] - (Trastuzumab Vedotin, Research Grade)

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  • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?

    A: A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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