Erythropoietin R Antibody (3D10) - Azide and BSA Free

Novus Biologicals | Catalog # H00002057-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3D10

Format

Azide and BSA Free
View all Erythropoietin R Primary Antibodies »

Product Specifications

Immunogen

EPOR (NP_000112, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PDPKFESKAALLAARGPEELLCFTERLEDLVCFWEEAASAGVGPGNYSFSYQLEDEPWKLCRLHQAPTARGAVRFWCSLPTADTSSFVPLELRVTAASGA

Specificity

EPOR - erythropoietin receptor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Erythropoietin R Antibody (3D10) - Azide and BSA Free

Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01]

Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01]

Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - Analysis of EPOR expression in transfected 293T cell line by EPOR monoclonal antibody (M01), clone 3D10.Lane 1: EPOR transfected lysate (Predicted MW: 55.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01]

Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01]

Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - Western Blot analysis of EPOR expression in different cell lines.
Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01]

Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01]

Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - EPOR monoclonal antibody (M01), clone 3D10. Analysis of EPOR expression in HeLa.
Immunoprecipitation: Erythropoietin R Antibody (3D10) [H00002057-M01]

Immunoprecipitation: Erythropoietin R Antibody (3D10) [H00002057-M01]

Immunoprecipitation: Erythropoietin R Antibody (3D10) [H00002057-M01] - Analysis of EPOR transfected lysate using anti-EPOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EPOR MaxPab rabbit polyclonal antibody.
ELISA: Erythropoietin R Antibody (3D10) [H00002057-M01]

ELISA: Erythropoietin R Antibody (3D10) [H00002057-M01]

ELISA: Erythropoietin R Antibody (3D10) [H00002057-M01] - Detection limit for recombinant GST tagged EPOR is approximately 0.03ng/ml as a capture antibody.

Applications for Erythropoietin R Antibody (3D10) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reviewed Applications

Read 1 review rated 5 using H00002057-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Erythropoietin R

The erythropoietin receptor is a member of the cytokine receptor family. Upon erythropoietin binding, the erythropoietin receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis.

Long Name

Erythropoietin Receptor

Alternate Names

EpoR

Entrez Gene IDs

2057 (Human)

Gene Symbol

EPOR

UniProt

NP_000112

Additional Erythropoietin R Products

Product Documents for Erythropoietin R Antibody (3D10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Erythropoietin R Antibody (3D10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Erythropoietin R Antibody (3D10) - Azide and BSA Free

Customer Reviews for Erythropoietin R Antibody (3D10) - Azide and BSA Free (1)

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  • Erythropoietin R Antibody (3D10)
    Name: zhang meng
    Application: Western Blot
    Sample Tested: HASMC human aortic smooth muscle cells
    Species: Human
    Verified Customer | Posted 11/17/2017
    Erythropoietin R Antibody (3D10) - Azide and BSA Free H00002057-M01

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FAQs

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail