ESRP1 Antibody (27H12.F3.H5) - BSA Free

Novus Biologicals | Catalog # NBP1-42778

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Human (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 27H12.F3.H5

Format

BSA Free
View all ESRP1 Primary Antibodies »

Product Specifications

Immunogen

ESRP1 Antibody (27H12.F3.H5) was produced by repeated immunizations of full length recombinant mouse ESRP1 fusion protein. (Uniprot: Q3US41)

Reactivity Notes

A BLAST analysis of the immunizing protein sequence shows 100% homology with ESRP1 from mouse and a 91% sequence homology with ESRP1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.

Specificity

This antibody reacts with mouse ESRP1 protein. A BLAST analysis of the immunizing protein sequence shows 100% homology with ESRP1 from mouse and a 91% sequence homology with ESRP1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This product is an IgG fraction antibody purified by Protein-A chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for ESRP1 Antibody (27H12.F3.H5) - BSA Free

Western Blot: ESRP1 Antibody (27H12.F3.H5) [NBP1-42778]

Western Blot: ESRP1 Antibody (27H12.F3.H5) [NBP1-42778]

Western Blot: ESRP1 Antibody [NBP1-42778] - Detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature.

Applications for ESRP1 Antibody (27H12.F3.H5) - BSA Free

Application
Recommended Usage

ELISA

1:750000

Western Blot

1:1000
Application Notes
This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: ESRP1

Epithelial splicing regulatory protein 1 (ESRP-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). ESRP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Alternate Names

epithelial splicing regulatory protein 1, FLJ20171, RBM35A, RMB35A, RNA binding motif protein 35A, RNA-binding motif protein 35A, RNA-binding protein 35A

Entrez Gene IDs

207920 (Mouse)

Gene Symbol

ESRP1

UniProt

Q3US41 (Mouse)

Additional ESRP1 Products

Product Documents for ESRP1 Antibody (27H12.F3.H5) - BSA Free

Certificate of Analysis

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Product Specific Notices for ESRP1 Antibody (27H12.F3.H5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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