ETHE1 Antibody (001) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90300

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ETHE1 (Uniprot#: O95571; Leu13-Ala254).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ETHE1 Antibody (001) - Azide and BSA Free

Western Blot: ETHE1 Antibody (001) [NBP2-90300]

Western Blot: ETHE1 Antibody (001) [NBP2-90300]

Western Blot: ETHE1 Antibody (001) [NBP2-90300] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:28 kDa
Immunoprecipitation: ETHE1 Antibody (001) [NBP2-90300]

Immunoprecipitation: ETHE1 Antibody (001) [NBP2-90300]

Immunoprecipitation: ETHE1 Antibody (001) [NBP2-90300] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-ETHE1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ETHE1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 28 kDa

Applications for ETHE1 Antibody (001) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ETHE1

ETHE1 encodes a protein that is expressed in the thyroid. [provided by RefSeq]

Alternate Names

D83198, EC 3.1.2.6, ethylmalonic encephalopathy 1, Ethylmalonic encephalopathy protein 1, Hepatoma subtracted clone one protein, HSCOEC 3.-, protein ETHE1, mitochondrial, YF13H12

Gene Symbol

ETHE1

Additional ETHE1 Products

Product Documents for ETHE1 Antibody (001) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ETHE1 Antibody (001) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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