EXOC2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EXOC2 Antibody [NBP1-83786]

Immunocytochemistry/Immunofluorescence: EXOC2 Antibody [NBP1-83786] - Staining of human cell line U-251 MG shows positivity in vesicles.

Western Blot: EXOC2 Antibody - BSA Free [NBP1-83786] -

LAMP2A KO leads to the down-regulation of KFERQ-containing proteins in sEVs.(A) Schematic representation of LAMP2 genomic sequence. There are three isoforms of LAMP2 (A, B, and C) originated by the alternative splicing of exon 9. In red is the exon for the LAMP2A isoform. ARPE-19 cells were cultured in exosome-depleted medium. sEVs were isolated from cell culture supernatants. (B) TEM images show isolated exosomes. Graphs show particle number and size using nanoparticle tracking system (NanoSight). LAMP2A KO maintains particle size and number. (C) WB of cell extracts and sEV fractions blotted with antibodies raised against membrane EV-positive markers (CD63 and LAMP2A), cytosolic EV-positive markers (HSC70 and FLOT1), EV-negative markers [CANX (calnexin) and ATP1A1 (ATPase Na+/K+ Transporting Subunit Alpha 1)], and KFERQ-containing proteins. LAMP2A KO leads to a decrease in the protein levels of EEA1, EXOc2, and histone 3, which contain at least one KFERQ motif each, as well as HSC70. (D) Raw MS/MS fragmentation spectrum of the peptide HHHAGYEQF, exclusively from LAMP2 isoform A, present only in WT samples. (E) Schematic representation shows the rules for the identification of putative KFERQ motifs. Graph shows the percentage of proteins with at least one KFERQ motif in their sequence that is present in sEVs. (F) Heatmaps of down-regulated proteins in sEVs after LAMP2A KO. Down-regulated proteins (67%) contain KFERQ motifs. All samples were analyzed under the same experimental conditions. The results represent means +/- SD of at least n = 3 independent experiments (***P < 0.001 and ****P < 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35333565), licensed under a CC-BY license. Not internally tested by Novus Biologicals.